RESEARCH

Severe mitochondrial damage affects cell fitness and viability by energy depletion, and very often, accompanied with increased oxidative stress. We are interested in whether mitochondrial damage also affects cell fitness and survival by non-bioenergetic mechanisms. Our studies revealed that mitochondrial protein import is readily saturable in the cell. Our genetic studies suggested that mitochondrial protein import is affected by a variety of mitochondrial stressors (e.g., protein misfolding, mtDNA mutation and low membrane potential) without directly disrupting the core protein import machinery. More importantly, biochemical analysis demonstrated that the cytosol has a limited capacity to degrade unimported mitochondrial proteins. Consequently, mitochondrial damage leads to the toxic accumulation of unimported mitochondrial proteins in the cytosol. We phenotypically and biochemically captured the cytosolic stress caused by unimported mitochondrial proteins, which we named mitochondrial Precursor Over-accumulation Stress (mPOS). The overall implication of these findings is that mitochondrial damage can directly cause proteostatic stress in the cytosol. This can induce cell death independent of bioenergetic defect. Using cell-based approaches, we are interested to determine the mechanisms by which mPOS modulates tissue function and causes cell death, and to identify molecular pathways that protect cells against mPOS.

Role of mPOS in muscle wasting, cardiac function and neurodegeneration

Mitochondrial abnormalities and cytosolic protein misfolding are probably the two most important hallmarks of aging and aging-associated degenerative disorders that include Parkinson's disease, amyotrophic lateral sclerosis, frontotemporal dementia, Alzheimer's disease and certain subtypes of muscle atrophies. The mPOS hypothesis provides a conceptual framework for untangling the interaction between these two ostensibly unrelated pro-degenerative pathways. We are interested to learn how mPOS might affect muscle mass homeostasis, cardiac function and neuronal health.